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Through the lens of science, we develop clear solutions.

2 UNIVERSITIES
4 YEARS OF R&D
37 RESEARCHERS
1000+ PUBLICATIONS
9.000+ HOURS OF RESEARCH

Deep dive on the issue

Problem-solving is fundamental to human nature, as it is essential for our ability to thrive. Science plays a critical role in problem-solving, providing a systematic and evidence-based approach to understanding and explaining the natural world around us.

Our scientific methods are based on observation and identification of a problem through data collection. We analyze the problem and use literature research and randomised controlled trials to evaluate potential nutrients that address a specific issue.

The literature used had to meet the following requirements: studies performed on humans, English or Dutch studies, peer-reviewed articles, (systematic) reviews, meta-analyses, clinical trials, safety, and randomized controlled trials (RCTs).

Scope

01 Before

Muscle fatigue and losing muscle endurance during activity are problems that seriously impair performance. It not only affects overall performance but eventually decreases aerobic capacity, flexibility, muscular strength, endurance and speed. We researched:

  • The physiological needs of athletes pre-activity.
  • The function, effects en synergy between potential ingredients.
  • Usability and safety of the potential ingredients.

02 During

Both energy management and thermoregulation are important during exercise, especially for team-, and endurance athletes. Not being on top of your strategy can result in gastrointestinal problems, muscle cramps, fatigue and (mental) exhaustion, all leading to sub-optimal performance when not addressed properly. We researched:

  • The current sports nutrition and supplement usage, and eating habits of athletes during activity.
  • The most important pharmacokinetic and pharmacodynamic properties of identified nutrients.
  • The main key problems regarding gastrointestinal and digestive issues, and usability.

03 After

During exercise or racing, athletes often experience significant exercise-induced muscle damage (EIMD) and wear and tear. If not addressed appropriately, inadequate recovery can lead to more severe injuries affecting various parts of the body, including muscles, bones, joints, and connective tissues. Beyond injury prevention, proper post-activity nutrition supports lean muscle growth and maintenance. Our research revealed:

  • The effects of the different ingredients in 03 After on sports recovery and how they co-interact.
  • Physiological mechanisms of wear and tear, and nutritional factors.
  • The nutrients that support recovery, replenishment and injury prevention, and the underlying mechanisms, as they are complementary.
How to use Our products are designed to optimise your sports nutrition intake. No essential nutrients are left out, and timing and planning are as easy as it should be.

01 Before: use 30 min. before your training or race.
02 During: depending on duration and intensity, you either use one (45 g/h), or two units (90 g/h). Trained, professional athletes can use up to 3 units per hour.
03 After: regardless of intensity or duration, use within 30 min. after training/race.

Publications

Showing 1 to 20 of 1128 Publications

2010
Astrocytic adrenoceptors and learning: α1-Adrenoceptors
2013
Albumin synthesis in surgical patients
2005
Evening intake of α-lactalbumin increases plasma tryptophan availability and improves morning alertness and brain measures of attention
2015
Changes in serum free amino acids and muscle fatigue experienced during a half-ironman triathlon.
1998
Muscle Amino Acid Metabolism at Rest and during Exercise: Role in Human Physiology and Metabolism.
2008
Amino acid transport across mammalian intestinal and renal epithelia
2018
Metabolism of Amino Acids. In Human Biochemistry (pp. 359–394).
2007
CE methods for the determination of non-protein amino acids in foods.
1991
Plasma and muscle amino acid and ammonia responses during prolonged exercise in humans.
1996
Effects of an Amino Acid-Carbohydrate Drink on Exercise Performance After Consecutive-Day Exercise Bouts
2006
Amino Acid, Mineral and Fatty Acid Content of Pumpkin Seeds (Cucurbita spp) and Cyperus esculentus Nuts in the Republic of Niger
2000
Effects of Exercise Training and Amino-Acid Supplementation on Body Composition and Physical Performance in Untrained Women
1999
Nonessential amino acids are not necessary to stimulate net muscle protein synthesis in healthy volunteers
2010
Bioavailability of anthocyanins and ellagitannins following consumption of raspberries by healthy humans and subjects with an ileostomy
1999
Anthocyanins in aged blueberry-fed rats are found centrally and may enhance memory
2022
Current knowledge of anthocyanin metabolism in the digestive tract: absorption, distribution, degradation, and interconversion
2021
Pharmacokinetic and excretion study of Aronia melanocarpa anthocyanins bound to amylopectin nanoparticles and their main metabolites using high- performance liquid chromatography-tandem mass spectrometry
2017
A theoretical physiologically based pharmacokinetic approach for modeling the fate of anthocyanins in vivo
2020
A 90 day oral toxicity study of blueberry polyphenols in ovariectomized sprague-dawley rats
2006
Concentrations of anthocyanins in common foods in the United States and estimation of normal consumption

Trusted by elite athletes

Matthijs Büchli

Matthijs Büchli is a Dutch road and track cyclist, who currently rides for UCI Track Team BEAT Cycling. He became world champion in the keirin at the 2018 UCI Track Cycling World Championships. In 2016, Büchli won the silver medal in the men's keirin at the Olympic Games in Rio de Janeiro, despite experiencing a severe knee injury only five months before the Olympic Games. Büchli was also a part of the team that won the gold medal at the 2020 Summer Olympics in the team sprint event.

Bas Diederen

Bas Diederen is a renowned triathlete hailing from the Netherlands. He has made a significant mark in the world of triathlon with 45 starts throughout his career. Among these, he has secured a place on the podium three times and has celebrated victory once. Some of his notable achievements include his performance at the 2019 NED Sprint Triathlon National Championships and the 2013 NED Triathlon National Championships. Representing the Netherlands on various international platforms, Diederen has showcased his dedication and prowess in the sport, making him a prominent figure in the triathlon community.

Ella Grapperhaus

Ella Grapperhaus has achieved notable titles such as the Enfusion World Champion at 54 kilos, European ISKA Champion at 56 kilos, and a two-time Power Women Title holder. Ella's passion for kickboxing was ignited after an unexpected fight in Dubai. Trained at Ettaki Gym under the guidance of Oussama and Hassan Ettaki, she emphasizes the importance of hard work, motivation, and the team dynamic between a fighter and their trainer. Apart from her fighting career, Ella is a weight consultant and advocates for a natural, healthy diet. She envisions a future connected to the sport, possibly through her own gym.

01 Before
starting at €33
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01 Before consists of a carefully balanced blend of ergogenic nutrients that enhance your performance and delay fatigue during activity. The composition of 01 Before provides the right amount of energy and focus that you maintain throughout your workout, and without crashing afterwards.
02 During
starting at €33
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Whether you're a professional, fanatic or enthusiast, 02 During is a very effective, gut-friendly and easy-to-use intra-activity high-carb solution. 

Containing 45 g of carbohydrates and 343 mg sodium, 02 During is unrivalled in effectiveness and the ideal choice for high-intensity or endurance athletes.

Available as drink mix and gel.
03 After
starting at €39
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03 After contains 34 different active nutrients, which all play a crucial role in full-body recovery. 

03 After focuses on muscle recovery as well as repairing tendons, ligaments, joints, blood sugar levels and more, due to a rich, complex mix of both macro-, and micro-nutrients, all perfectly weighted and balanced.